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Experimental approaches—CC50 calculation of phenformin and atpenin A5 CC 50 calculations were performed using three methods to measure cell viability: the MTT assay, the CellTiter-Glo® assay (CTG) from Promega (Cat# G7570), and monitoring cell viability via live cell imaging (IncuCyte®)
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**What it doesn't show:** - Human pharmacokinetics, bioavailability, or appropriate dosing - Safety in humans at any dose — including immunogenicity risk, which is a real concern with D-amino acid peptides that the immune system may not process normally - Efficacy for any specific human condition - Long-term effects of selectively eliminating senescent cells, which play roles in wound healing and tumor suppression in addition to their negative effects in aging tissue[3] - Whether the FOXO4–p53 interaction in human senescent cells behaves identically to the mouse model **Pending regulatory decisions:** The FDA has not announced any plans to evaluate FOX04-DRI for approval or to add it to compounding bulk substance lists
